Ohtsuka Masato

論文

Targeted insertion of conditional expression cassettes into the mouse genome using the modified i-PITT

CRISPR-KRISPR: a method to identify on-target and random insertion of donor DNAs and their characterization in knock-in mice.

Prototype mouse models for researching SEND-based mRNA delivery and gene therapy.

Novel reporter mouse models useful for evaluating in vivo gene editing and for optimization of methods of delivering genome editing tools.

Creation of CRISPR-based germline-genome-engineered mice without ex vivo handling of zygotes by i-GONAD.

i-GONAD: a robust method for in situ germline genome engineering using CRISPR nucleases.

Easi-CRISPR for creating knock-in and conditional knockout mouse models using long ssDNA donors

Easi-CRISPR: A robust method for one-step generation of mice carrying conditional and insertion alleles using long ssDNA donors and CRISPR ribonucleoproteins

One-step generation of multiple transgenic mouse lines using an improved Pronuclear Injection-based Targeted Transgenesis (i-PITT)

Pronuclear injection-based mouse targeted transgenesis for reproducible and highly efficient transgene expression

共同研究・競争的資金等の研究課題

Analysis of genomic DNA in aging heart

Development of Organ- and Cell-specific In Vivo Genome Editing Technique as a Novel Approach for Cancer Gene Therapy

Development of Organ- and Cell-specific In Vivo Genome Editing Technique as a Novel Approach for Cancer Gene Therapy

Determination of chronic or intractable mechanisms induced by low bone quality with osteomacs and bone marrow derived stem cells interaction

Establishment of a next-generation impact assessment tool for low-concentration chemical exposure using THA rats

Understanding of pathophysiology and development of treatment strategy for MRONJ based on the hierarchical structure of macrophages

Understanding of pathophysiology and establishment of new treatment strategy by determining the hierarchy of macrophages in medication-related osteonecrosis of the jaw

Establishment of gene expression control system by modification of introns and its application to analysis of diabetes-related gene functions

Establishment of gene expression system by intron modification and its application for the functional analysis of diabetes-related genes

Development of novel genome editing tool for gene therapy and its application to cancer therapy

Generation of gene modified mice using in situ genome editing system without microinjection

Molecular targeted therapy for MRONJ

Identification of miRNA which rescues EBF1 deficiency indispensable for B cell lineage commitment